Figure 9

Strong resistance of Il17c^−/− mice to T cell-independent endotoxin shock. Female mice were injected intraperitoneally with LPS. After LPS injection, survival was monitored, and peritoneal fluids were collected at the indicated points. (a) Survival of wild-type (n = 47) and Il17c^−/− (n = 46) mice after LPS injection. ***p < 0.001. (b) The concentrations of IL-17C, IL-1β, IL-6, IL-17A and TNF in peritoneal lavage fluids from wild-type mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) and Il17c^−/− mice (0 h, n = 5; 3 h, n = 5; 6 h, n = 6) were measured by ELISA. The data show the mean + SEM. *p < 0.05: wild-type mice vs. Il17c^−/− mice. (c) Peritoneal F4/80⁻ and F4/80⁺ cells were stimulated with LPS for the indicated times. The expression levels of Il17c and Il17re mRNAs in the cells were determined by quantitative PCR. The data show the mean + SEM (n = 3). (d) The expression levels of Il17c, Il17re, Il1b, Il6, Il17a and Tnfa mRNAs in the tissues and peritoneal lavage fluid cells from wild-type mice at 0, 3 and 6 h after LPS injection were determined by quantitative PCR. The data show the mean + SEM (n = 5). *p < 0.05, **p < 0.01 and ***p < 0.001 vs 0 h (c,d).