Figure 3

Cecal bacterial composition. (A) Mean proportional abundance of bacterial sequencing data at phylum level (n = 12). Centre lines show the medians and box plot limits indicate the 25^th and 75^th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25^th and 75^th percentiles, individual animals are presented by dots. (B) Bray Curtis cluster dendrogram of bacterial composition at family level in mice fed mice fed a high fat diet (HFD, red), HFD where 10% of the carbohydrate by weight (5% corn starch, 5% cellulose) was replaced by beta glucan (HFD + bglucan, dark green), apple pectin (HFD + pectin, light green), inulin (HFD + inulin, purple), inulin acetate ester (HFD + inul A, blue), inulin propionate ester (HFD + inul P, light blue), inulin butyrate ester (HFD + inul B, dark blue), inulin propionate and butyrate ester, 5% each (HFD + inul PB, blue-green) and low fat diet (LFD, orange). Also see high resolution of Fig. 3B in Supplementary File S3 (C) Heat map of relative abundance (expressed as average percentage to total sequences per diet) of the 38 most abundant OTUs (≥0.5% of total sequences; data for all OTUs are given in Supplementary File S4). (D) Total universal 16S rRNA gene copies in cecal content. Centre lines show the medians and box plot limits indicate the 25^th and 75^th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25^th and 75^th percentiles, individual animals are presented by dots.