Figure 4 | Scientific Reports

Figure 4

From: Dietary fibers inhibit obesity in mice, but host responses in the cecum and liver appear unrelated to fiber-specific changes in cecal bacterial taxonomic composition

Figure 4

Validation of microarray data and responses in mice fed a high fat diet (HFD), where 10% of the carbohydrate by weight (5% corn starch, 5% cellulose) was replaced by beta glucan (HFD + bglucan), apple pectin (HFD + pectin), inulin (HFD + inulin), inulin acetate ester (HFD + inul A), inulin propionate ester (HFD + inul P), inulin butyrate ester (HFD + inul B), inulin propionate and butyrate ester, 5% each (HFD + PB) and low fat diet (LFD). (A) RT Profiler PCR Array of selected gene targets showing altered gene regulation in response to HFD + inul and HFD + PB from microarray data analysis of cecum. Fold change was calculated relative to HFD fed mice using mean gene target normalised to UBE2D2 (n = 6). (B) Enho gene expression in liver of mice fed HFD + DF or LFD relative to HFD. Fold change was calculated relative to HFD fed mice using mean Enho normalised to UBE2D2 (n = 6). (C) Adropin levels in liver. A Student’s t-test based on delta CT values was applied to test comparisons with HFD fed mice. *P < 0.05, **P < 0.01, ***P < 0.001.

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