Figure 6
From: The Sema3A receptor Plexin-A1 suppresses supernumerary axons through Rap1 GTPases
Active Rap1B recues the suppression of axon formation by constitutively active Plexin-A1. (a) Hippocampal neurons from E18 rat embryos were transfected with vectors for GFP (green) and Plexin-A1Δsema (PlxnA1-CA), Rap1BV12 or a combination of both and analyzed at 3 d.i.v. by staining with an anti-MAP2 (red, dendrites) and the Tau-1 antibody (blue, axon). pBK-CMV and pSHAG-1 were transfected as control. Representative images of transfected neurons are shown. The scale bar is 25 μm. (b) The percentage of unpolarized neurons without an axon (0, red), polarized neurons with a single axon (1, blue) and neurons with multiple axons (>1, green) is shown (Student’s t-test and two-way ANOVA; n = 3, independent experiments with >150 neurons per experiment; values are means ± s.e.m, **p < 0.01; ***p < 0.001 compared to control and as indicated).
