Figure 6

Immunohistofluorescence analysis pAMPK, IL-1β and NFkB proteins in rat brain 2, 8 and 12 weeks after surgery. The double immunohistofluorescence images of pAMPK/NFkB and IL-1β/NFkB in rat brain (A) 2 weeks, (B) 8 weeks, and (C) 12 weeks after surgery. The blue color represents nuclear staining (DAPI) while red (NFkB) and green (pAMPK and IL-1β) represent Alexa Fluor and FITC staining of secondary antibodies, respectively. Scale bar = 100 µm. For immunohistofluorescence analysis, 10–14 µm transverse sections of the forebrain region were made. Each picture contains part of the gray cortex, cerebral cortex and white matter.