Figure 2
From: Direct visualization of DNA baton pass between replication factors bound to PCNA
Sample preparation of FEN-PCNA-DNA (flap). (a) Schematic diagram of the FEN-PCNA-DNA complex with the DNA sequences used for the reconstitution. (b) Gel filtration chromatography of the reconstituted FEN-PCNA-DNA complex. The absorbances at 260 and 280 nm are indicated by red and blue lines, respectively. (c) The peak fractions indicated by the asterisk (*) in (b) were analyzed by gradient (10–20%) SDS-PAGE (lane 1–3). The lanes for the purified proteins used for complex reconstitution and the markers are indicated by the notations “PCNA”, “FEN”, and “Marker”, respectively.
