Figure 4
From: Direct visualization of DNA baton pass between replication factors bound to PCNA
Sample preparation of FEN-Lig-PCNA-DNA (nick). (a) Schematic diagram of the FEN-Lig-PCNA-DNA complex with the DNA sequences used for the reconstitution. (b) Gel filtration chromatography of the reconstituted FEN-Lig-PCNA-DNA complex. (c) The peak fractions indicated by the asterisks (* and **) in (a) were analyzed by gradient (10–20%) SDS-PAGE (lane 4–7 and 10). Proteins corresponding to the bands are labeled on the left. Molecular weight standards are shown on the right.
