Figure 5

3D structure of the P. furiosus FEN-Lig-PCNA-DNA complex. (a) Bottom view, (b) top view, (c) side view, and (d) oblique view of the class 1 complex. The map is colored according to the components and domains. The fitted P. furiosus FEN and P. furiosus PCNA crystal structures and the atomic model of DNA are shown in sky-blue, green, and pink ribbons, respectively. The crystal structures of the P. furiosus Lig domains, DBD (orange), AdD (light orange), and OBD (yellow), were docked independently into the map. In (c), the PIP-box motif (¹⁰³QKSFF¹⁰⁷) is highlighted in red and the IDCL of PCNA is indicated with a dashed line. (e) Bottom view, (f) top view, (g) side view, and (h) oblique view of the class 2 complex. The ribbon models are colored as (a–d).