Figure 2
c-Jun/JunB knock-down in cHL cell lines results in a reduced growth rate. (A–F) Growth curves examining the effect of c-Jun (A–C) or JunB (D–F) knock-down in cHL cell lines expressing the indicated shRNAs. The results represent the average and standard deviation of four (A,B), five (C,D,F) or three (E) independent experiments from two separate infections. Note: the control shRNA data in (C,F) is the same because c-Jun and JunB knock-down cells were examined together in the same experiments. (G) Western blots showing c-Jun and JunB levels in L-428 cells expressing the indicated shRNAs used in the mouse xenograft experiments. Note: the two blots are the same lysates run on one gel that was cut prior to probing. Molecular mass markers (in kDa) are indicated to the left of western blots. (H) Ten mice were injected with each of the shRNA–expressing cell lines shown in (G). Fifty-nine days after injection, mice were euthanized and tumours were excised. Pictures showing representative median sized tumours from each group (I), and plot showing the weight (in mg) of tumours isolated from mice (J). The average tumour weight in each group is represented by the horizontal line and the open dot indicates the corresponding tumour in (I). The results of the mice xenograft experiments are representative of two independent experiments. Note: in the experiment shown one mouse in the control shRNA group was euthanized prior to day 59 because the tumour had reached an unacceptable size, and we could not find the tumour in one mouse in the JunB shRNA group. P values were obtained by performing ANOVA with Tukey’s post hoc test comparing controls to c-Jun/JunB shRNA–expressing cells with the exception that a two-tailed t test was performed in (F). comparing control to JunB shRNA–expressing cells. ns; not significant, *P < 0.05, **P < 0.01, ***P < 0.001. In (A,B), the first P value is the knock-down compared to control 002 and the second is compared to control 216.
