Figure 6
Dysregulation of G1 regulators in c-Jun/JunB knock-down cell lines. Western blots demonstrating the differential expression of p21Cip1/Waf1 protein levels in L-540 (A) and L-428 (B) cHL cell lines expressing the indicated shRNAs. In (B), the two membranes with control and c-Jun#1 samples are the same lysates run on two different gels. (C,D) Western blots comparing the expression of the indicated cell cycle regulators between control and JunB shRNA–expressing Karpas 299 (C) and SUP-M2 (D) cells. In both (C,D), each vertical column of blots represents the same lysates run out on different gels. Molecular mass markers (in kDa) are indicated to the left of western blots. qRT-PCR data examining p21Cip1/Waf1 (E) or p27Kip1 (F) mRNA expression in the indicated cell lines. The results represent the average and standard deviation of at least 3 independent experiments from at least two infections. P values were obtained by performing ANOVA with Tukey’s post hoc test when c-Jun/JunB knock-down cells with two shRNAs with control shRNA–expressing cells or independent, two-tailed t tests when comparing JunB knock-downs with one shRNA to control shRNA–expressing cells.
