Table 1 c-Jun or JunB knock-down in cHL cell lines is associated with a prolonged G0/G1.

From: The c-Jun and JunB transcription factors facilitate the transit of classical Hodgkin lymphoma tumour cells through G1

L-540

Control 002

Control 216

c-Jun#1

c-Jun#4

Doubling time (h)

29

34

40

43

G0/G1 (h)

14.1 +/− 1.2

16.5 +/− 1.8

23.1 +/− 3.1***/***

25.7 +/− 1.8***/***

S (h)

12.5 +/− 1.3

15.1 +/− 2.2

14.0 +/− 2.8*

13.8 +/− 2.4

G2/M (h)

2.4 +/− 0.4

2.5 +/− 0.4

3.0 +/− 0.7

3.5 +/− 1.2

L-428

Control 002

Control 216

c-Jun#1

c-Jun#2-1

Doubling time (h)

36

37

42

49

G0/G1 (h)

14.9 +/− 0.6

15.1 +/− 0.6

21.3 +/− 0.4***/***

25.2 +/− 0.8***/***

S (h)

16.6 +/− 0.8

16.9 +/− 0.8

16.5 +/− 1.4

19.5 +/− 0.8**/*

G2/M (h)

4.5 +/− 0.8

5.0 +/− 1.0

4.4 +/− 1.7

4.3 +/− 1.5

L-428

Control 216

JunB#1

JunB#5

 

Doubling time (h)

34

37

40

 

G0/G1 (h)

16.5 +/− 1.7

19.3 +/− 1.4**

21.3 +/− 1.9***

 

S (h)

14.7 +/− 1.2

13.3 +/− 1.7

13.8 +/− 1.9

 

G2/M (h)

2.8 +/− 0.8

4.5 +/− 0.4*

4.8 +/− 0.6**

 

KM-H2

Control 216

c-Jun#1

c-Jun#2-3

JunB#1

Doubling time (h)

28

35

34

31

G0/G1 (h)

12.3 +/− 1.0

17.7 +/− 2.0***

17.7 +/− 1.8***

14.6 +/− 1.4*

S (h)

13.7 +/− 1.1

14.7 +/− 2.2

13.6 +/− 1.6

14.2 +/− 1.6

G2/M (h)

2.0 + /−0.6

2.7 +/− 1.0

2.8 +/− 0.8

2.3 +/− 0.5

  1. Approximate doubling time and time spent in each stage of the cell cycle was determined for L-540, L-428 and KM-H2 cells expressing control, c-Jun or JunB shRNA as described in the Methods. The results represent the average and standard deviation of at least four independent experiments. P values were obtained by performing ANOVA with Tukey’s post hoc test comparing the c-Jun/JunB knock-down cells with control shRNA–expressing cells. When two P values are given, the first is compared to control 002 and the second is to control 216. *P < 0.05, **P < 0.01, ***P < 0.001.
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