Figure 9

Enalapril treatment blocks activation of the TGF-β signaling pathway in UUO-CKD mice. (A) qRT-PCR revealed that Enalapril treatment reduced the expression of TGF-β mRNA in the hearts of UUO/CKD mice. The expression level of TGF-β was normalized to the expression level of β-actin. *P < 0.05 vs. Control, ###P < 0.001 vs. UUO. (B) Immunoblotting revealed that Enalapril treatment blocked UUO-induced phosphorylation of Smad2 and Smad3 in the hearts of UUO/CKD mice. Data were normalized to total Smad2/3. **P < 0.01 vs. Control, #P < 0.05 vs. UUO, ###P < 0.005 vs. UUO. Values were expressed as means ± SEM (error bars). Statistical analyses were performed with one-way ANOVA. N = 6.