Figure 3
From: Fibroblast Growth Factor Binding Protein 3 (FGFBP3) impacts carbohydrate and lipid metabolism
Chronic BP3 expression induces hepatic Stat3 and Akt activation and modulates glucose and fat enzymatic cascades in ob/ob mouse livers and WAT. (A) Schematic diagram showing the protocol for the in vivo transfection of an mBP3 expression vector in ob/ob mice. (B,C) Changes in liver (B) and WAT (C) gene expression analyzed by qRT-PCR in mBP3 transfected ob/ob mice (n = 6). Values are calculated as fold of control (n = 3). (D) Stat3 and Akt phosphorylation in livers from mBP3 transfected ob/ob mice or control (n = 3) analyzed by immunoprecipitation (IP) and western blot (WB). Fold activation over controls is shown. (E) Schematic diagram showing the in vivo delivery of a single dose of an mBP3 expression vector in ob/ob mice. Mice were euthanized 72 hours post treatment. (F) Random blood glucose levels at 0, 24, 48 and 72 hours post treatment in control and mBP3 transfected fed ob/ob mice. (G,H) Change in gene expression in ob/ob mouse livers (G) and WAT (H) measured by qRT-PCR at the times indicated after a single delivery of mBP3 or control DNA (n = 4). Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.0001.
