Figure 3 | Scientific Reports

Figure 3

From: Triple herbal extract DA-9805 exerts a neuroprotective effect via amelioration of mitochondrial damage in experimental models of Parkinson’s disease

Figure 3

DA-9805 restores mitochondrial activity. SH-SY5Y cells were placed in a 96-well plate and treated with DA-9805 (μg/ml) or reference drugs (50 µM creatine, 100 nM rasagiline, or 50 µM ropinirole) for 4 h. The cells were incubated with 1 mM MPP+ for 20 h, and the mitochondrial activity was analyzed. (a) Intracellular ATP content. (b) TMRE-mediated mitochondrial membrane potential. (c) MitoSox-based mitochondrial superoxide generation. (d) DCF-DA-based total reactive oxygen species (ROS) generation. All values are reported as a percentage of the control (CTL). The data are plotted as the mean ± SEM (n = 6). #p < 0.05, ##p < 0.01, ###p < 0.001 vs. CTR; *p < 0.05, **p < 0.01, and ***p < 0.001 vs. MPP+-treated DMSO. p values are from a one-way ANOVA followed by Tukey’s test.

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