Table 2 Protocol details and primers sequences.

From: The mitochondrial antioxidant SS-31 increases SIRT1 levels and ameliorates inflammation, oxidative stress and leukocyte-endothelium interactions in type 2 diabetes

qRT-PCR protocol

Temperature

95 °C

95 °C

60 °C

Melting

Time

10 min

10 s

30 s

curve

No. of Cycles

1

40

Primers

Target

Direction

Sequence (5′-3′)

Sirt1

Forward

TGGGTACCGAGATAACCTTCT TGTTCGAGGATCTGTGCCAA

Reverse

Gapdh

Forward

CGCATCTTCTTTTGCGTCG TTGAGGTCAATGAAGGGGTCA

Reverse

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