Figure 5
HD5 prevents DSS-colitis and EtOH-induced disruption of tight junction (TJ) and adherens junction (AJ) in mouse colon. Adult mice were subjected to DSS-induced colitis three times with 15-day intervals, followed by 30-day recovery from third colitis. During the intervals and recovery period, mice were fed a liquid diet with EtOH (EF) or isocaloric maltodextrin (PF) and with or without HD5 supplementation. Cryosections of colon were stained for occludin (green) and ZO-1 (red) (A) or E-cadherin (green) and β-catenin (red) (B) and the fluorescence images captured in a confocal fluorescence microscope. The white bars represent 8 μm in distance. Densities of ZO-1 (C) and β-catenin (D) fluorescence was measured. Values are mean ± sem (n = 3). Asterisks indicate the values that are significantly (p < 0.05) different from corresponding PF values, and the hash tags indicate the values significantly different from corresponding values for DSS without EtOH group. The symbol Δ indicates values that are different from corresponding values for DSS + EF group.
