Figure 3 | Scientific Reports

Figure 3

From: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program

Figure 3

Validation of amino acid transport, amino acid biosynthesis and tRNA charging gene expression. Messenger RNA expression of the following genes were validated by qPCR on all 164 samples (A) solute carrier family 3 member 2, (B) solute carrier family 7 member 1, (C) solute carrier family 1 member 5, (D) seryl-tRNA synthetase, (E) arginase 2, (F), argininosuccinate synthetase 1, (G) sestrin-2 and (H) cyclin-dependent kinase inhibitor 1A. *Indicates a treatment-time interaction with p < 0.001, $indicates a treatment-time interaction with p < 0.005, ^indicates a treatment effect with p < 0.01, &indicates a treatment effect with p < 0.05, b indicates a significant Dunnetts test with p < 0.01 for BA vs. control (GH vs. control  = NS). For genes with significant interactions, see Supplementary Table 2 for post hoc Bonferroni test comparisons. Data are mean ± SEM. Days 1, 3, 7 and 13 have n = 10 whilst day 27 has n = 15. White bars = control, black bars = GH (growth hormone), grey bars = BA (beta-adrenergic agonist).

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