Figure 2 | Scientific Reports

Figure 2

From: Genetic background and window of exposure contribute to thyroid dysfunction promoted by low-dose exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

Figure 2

Oxidative stress contributes to alteration of thyroid-enriched transcripts in FRTL5 exposed to TCDD. (a–c) RT-qPCR analysis of Nis transcript (a) and thyroid specific regulators Nkx2-1 (b) and Pax8 (c) in FRTL-5 cells treated with TCDD 10−9 M for 24 hrs. When reported, cells were co-treated with NAC 2 mM for 24 hr. (d) Western blotting analysis of nuclear/cytosolic localization of p65 protein in FRTL-5 untreated (CTRL) and exposed to 10−9 M TCDD. β-Tubulin and Topoisomerase 1 were used as loading control of cytoplasmic and nuclear fraction, respectively. A cropped version of the image is shown (full-lenght blots are shown in Supplementary Fig. S7). Cytoplasmic and nuclear fractions were loaded on two different gels. Cellular level of IκBα and Tp53 mRNAs (e and f, respectively) was assayed by RT-qPCR in CTRL- and TCDD-cells. Level of transcripts was determined after exposure to TCDD 10−9 M for 24 hr and reported as means ± SD of Gapdh normalized-mRNA levels of three independent experiments. *p-value < 0.05 compared with controls.

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