Figure 4 | Scientific Reports

Figure 4

From: Genetic background and window of exposure contribute to thyroid dysfunction promoted by low-dose exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

Figure 4

Characterization of thyroid function in mice with different genetic background or foetally exposed to TCDD. (a,b) ELISA assay of fT4 serum level was performed in C57BL/6 wild type (wt), Pax8+/− and Nkx2-1+/− mice (at left of the dashed lines) and in wt, Pax8+/− and Nkx2-1+/− treated with TCDD 0,1 μg/kg/day (at right of the dashed lines), males (a) and females (b). (c,d) Body weight analysis of wt, Pax8+/− and Nkx2-1+/− and TCDD-wt, -Pax8+/− and -Nkx2-1+/− mice, males (c) and females (d). (e,f) RT-qPCR analysis of Tsh in pituitary of wt, Pax8+/− and Nkx2-1+/− and TCDD-wt, -Pax8+/− and -Nkx2-1+/− mice, males (e) and females (f). Data are reported as means ± SD of Gapdh normalized-mRNA levels; n = 4 mice for each group. *p-value < 0.05; **p-value < 0.01 for statistical analysis of TCDD-exposed vs untreated mice having the same genotype; #p-value < 0.05; ##p-value < 0.01 TCDD-exposed vs TCDD-wt mice; ††p-value < 0.01 unexposed mice of different genotype vs wt mice.

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