Figure 6 | Scientific Reports

Figure 6

From: Genetic background and window of exposure contribute to thyroid dysfunction promoted by low-dose exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

Figure 6

Activation of NF-κB pathway in Pax8+/− or Nkx2-1+/− exposed mice. (a,c) Immune-peroxidase analysis of p65 protein in thyroids of CTRL wt, Pax8+/− and Nkx2-1+/− and of TCDD-wt, -Pax8+/− and -Nkx2-1+/−, males (a) and females (c) (n = 4 mice for each group and sex). Negative control was performed using purified IgG and it is shown in Fig. S3h. (b,d) RT-qPCR of IκBα gene in thyroid of C57BL/6 wild type (wt), Pax8+/− and Nkx2-1+/− (at left of the dashed lines) and in TCDD-wt, -Pax8+/− and -Nkx2-1+/− (at right of the dashed lines), males (b) and females (d). Data are reported as means ± SD of Gapdh normalized-mRNA levels; n = 4 mice for each group and sex. *p-value < 0.05 for statistical analysis of TCDD-exposed vs untreated mice having the same genotype; #p-value < 0.05 TCDD-exposed vs TCDD-wt mice; †p-value < 0.05 unexposed mice of different genotype vs wt mice.

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