Table 1 Substrate scope of VbTA T16F.

From: Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form

Amino donora

Conversion (%)

Amino acceptorb

Conversion (%)

43

<5

71

n.d.

47

49

95

94

70

View full size image

65

90

78

75

81

View full size image

86

View full size image

90

74

View full size image

93

90

91

n.d.

n.d.

86

88

n.d.

n.d.

n.d.

View full size image

n.d.

View full size image

n.d.

n.d.

  
  1. Each reaction was performed in triplicate and the results are reported as the average of the data obtained after 24 h. Substrates were prepared in methanol solution to guarantee the correct concentration of the substrate in the reaction mixture (final concentration 10% (v/v) MeOH). Final conversions were determined by HPLC. aAmino donor concentration was kept constant at 10 mM, and 10 mM pyruvate was used as the amino acceptor; bamino acceptor concentration was kept constant at 10 mM; L-alanine was used as the amino donor. n.d. = non-detectable in the tested condition.
Back to article page