Figure 1
From: Hornerin contains a Linked Series of Ribosome-Targeting Peptide Antibiotics
TEM analyses of HRNR-treated E. coli and P. aeruginosa. (a,b) Transmission electron microscopy (TEM) analyses of 6.25 × 107/ml E. coli ATCC 11775 in 10 mM NaP, pH 5.5, 1 h treatment with 312.5 µg/ml rHRNR2591–2684. (c) E. coli control. Note the absence of membrane perturbation and the presence of intracytoplasmic electron-dense aggregates in rHRNR2591–2684-treated bacteria (a,b). The hyperhydrated looking periplasmic space of many cells in the control (a), is similar as seen for E. coli treated with low ion strength and acidic buffers7. TEM of 6.25 × 107/ml P. aeruginosa ATCC 10145, in 10 mM NaP, pH 5.5, 1 h treatment with 312.5 µg/ml rHRNR2591–2684 (d,e). (f) P. aeruginosa control. Note condensation of electron-dense cytoplasmic material and blebs of the outer membrane with an occasional ballooning (d,e). 1 h treatment of 6.25 × 107/ml P. aeruginosa ATCC 10145 with 469 µg/ml rSUMO3-HRNR2591–2684 in 10 mM NaP, pH 5.5 revealed widespread peeling of the outer membrane (g,h). (i) P. aeruginosa control. Images are representative of two independent experiments, sampling on average 10 images per condition and species in each experiment.
