Figure 5
From: Hornerin contains a Linked Series of Ribosome-Targeting Peptide Antibiotics
HRNR binds to intracytoplasmic aggregates in P. aeruginosa. P. aeruginosa ATCC 10145 was treated for 5 min with rHRNR2591–2684 (45 µg/ml 10 mM NaP, pH 5.5), and then cellular localization of this CIDAMP was analyzed by immunocytochemistry with a HRNR2591–2684-specific polyclonal antibody, followed by incubation with a gold-conjugated secondary antibody (a). Bacteria, treated with rHRNR2591–2684 (b) or buffer (c), followed by incubation with the gold-conjugated secondary antibody, served as controls. Note accumulation of intracytoplasmic immuno-gold (a), which is corresponding to electron dense cytoplasmic aggregates seen upon TEM-analyses of CIDAMP-treated P. aeruginosa (Fig. 1c,d). Images are representative of two independent experiments, sampling on average 10 images in each experiment.
