Figure 4
From: Fast confocal fluorescence imaging in freely behaving mice
Optical characterization of the differential multipoint-scanning confocal modality. (A) Measured illumination power at the sample (dots) as a function of pinhole density D, normalized to the value measured for a density of 0.01. As expected from theory, illumination power is linear with D (solid blue line). (B) Signal measured from an in-focus fluorescent plane as a function of the pinhole size A and density D, for a constant illumination power of the sample. The signal is normalized to the maximum value, obtained for A = 12 μm and D = 0.04. (C) Linear plot of data in B) as a function of A, for D = 0.04. (D) Thickness of the optical section as a function of A and D. This thickness is calculated as the FWHM of sectioning curves similar to the solid black line in Fig. 3B. (E) Linear plot of data in D) as a function of A, for D = 0.04. In the range of pinhole sizes used in this work, section thickness is linear with the pinhole size (solid blue line).
