Figure 5
From: Fast confocal fluorescence imaging in freely behaving mice
Comparison of different techniques for imaging microvasculature in the cortex of anaesthetized mice following retro-orbital sinus injection of rhodamine dextran. (A–D) Differential (left) and regular (middle) multipoint-scanning confocal imaging are compared with conventional widefield imaging (right). Images are registered at depths of 10 μm (A), 40 μm (B), 70 μm (C) and 100 μm (D) in the brain. (E–H) Profiles along the yellow lines drawn in Figs (A–D) (respectively) measured with differential (solid black line) and regular (solid blue line) multipoint-scanning confocal imaging, and with conventional widefield imaging (dotted black line). Imaging speed: 100 Hz. A = 7.5 μm, D = 0.04.
