Figure 6
From: Fast confocal fluorescence imaging in freely behaving mice
Effect of the pinhole size for imaging microvasculature in anaesthetized mice with differential multipoint-scanning confocal microscopy. (A–C) Imaging close to the brain surface (z = 25 μm) with pinhole sizes of 4.5 μm (A) and 10.5 μm (B). (C) Profile along line (1) (shown on Figs A,B) measured with A = 4.5 μm (green line) and A = 10.5 μm (black line). The background at the out-of-focus vessel indicated by the blue arrow divided by the signal at the in-focus vessels indicated by the red arrows was 2x larger with A = 10.5 μm than with A = 4.5 μm. (D–G) Same as A-C for imaging far from the brain surface (z = 120 μm). (F) represents the profiles along line (2) and (G) along line (3). Imaging speed: 100 Hz. D = 0.04.
