Figure 7
From: Fast confocal fluorescence imaging in freely behaving mice
Measurement of RBC velocity in anaesthetized and awake mice. (A) Differential multipoint-scanning confocal image acquired in a freely behaving mouse. Exposure time: 5ms. A = 6 μm. D = 0.04. Time-lapse series is available as Supplemental Movie S1. (B) x-t profiles along the region of interest (ROI) indicated by the red arrow and shown in yellow on (A) Acquisition rates: 50 Hz (top) and 200 Hz (bottom). Dark streaks on the bottom profiles correspond to RBCs moving in the longitudinal direction, with a velocity directly equal to the slope of the streaks. These streaks could not be observed on the top profiles, showing that an acquisition rate of 50 Hz was too low for reliable extraction of the RBC velocity in this microvessel. (C) Comparison of RBC velocity in anaesthetized and in awake, freely behaving mice for 37 microvessels in 5 mice. (D) Histogram of the ratio of RBC velocities measured in awake versus anaesthetized mice.
