Figure 1 | Scientific Reports

Figure 1

From: Impact of Escherichia coli K12 and O18:K1 on human platelets: Differential effects on platelet activation, RNAs and proteins

Figure 1

P-selectin and CD63 expression on platelets after co-incubation with bacteria or platelet activators. P-selectin (a,b) or CD63 (c,d) were measured on the platelet surface by flow cytometry after gating for the presence of CD41. Platelets incubated without bacteria (PLT) and E. coli K12 or O18:K1 co-incubated platelets (platelet-bacteria ratios 1:1, 1:5, 1:10) were analysed at zero hours (a,c) and after three hours (b,d) incubation. The activating effect of bacteria was compared to platelet activation by TRAP, ADP or LPS after 15 minutes or three hours incubation time. The data represents percentages (mean ± standard error of the mean) from 3–6 independent experiments. Activation was compared to PLT controls, significance levels are: *p < 0.05, **p < 0.01, and ***p < 0.001. TRAP, thrombin receptor activating peptide 6; ADP, adenosine diphosphate; LPS, lipopolysaccharide.

Back to article page