Figure 2 | Scientific Reports

Figure 2

From: Impact of Escherichia coli K12 and O18:K1 on human platelets: Differential effects on platelet activation, RNAs and proteins

Figure 2

PAC-1 antibody and fibrinogen binding on platelet surface after co-incubation with E. coli K12 or O18:K1. PAC-1 antibody (a,b) and fibrinogen (c,d) binding were measured on the platelet surface by flow cytometry after gating for the presence of CD61. Platelets incubated without bacteria (PLT) and E. coli K12 or O18:K1 co-incubated platelets (platelet-bacteria ratios 1:1, 1:5, 1:10) were analysed at zero hours (a,c) and after three hours (b,d). TRAP treated platelets served as a positive control after 15 minutes or three hours incubation time. The data represents percentages (mean ± standard error of the mean) from three independent experiments. Activation was compared to PLT controls, significance levels are: *p < 0.05, **p < 0.01, and ***p < 0.001. TRAP, thrombin receptor activating peptide 6.

Back to article page