Figure 3

Changes of mRNAs in platelets after exposure to E. coli K12. Analysis with thromboSeq pipeline. (a) Heatmap of platelet mRNA profiles of control (grey) and E. coli K12 co-incubated platelets (green) after three hours incubation in 1:1 platelet-bacteria ratio. (b) Volcano plot of mRNAs of control versus E. coli K12 (1:1) exposed platelets. RNAs of which the concentrations changed significantly after E. coli K12 co-incubation are labelled in red (p < 0.05). (c) Gene ontology (GO) analysis of mRNAs with significantly changed concentrations after E. coli K12 exposure (p < 0.05) using DAVID functional annotation analysis (cluster enrichment score >1). (d) Logarithm 2 fold change (logFC) and normalized counts (norm. counts) of HMBS, ATP2C1 and LRCH4 RNAs in platelets exposed to E. coli K12 bacteria compared to control platelets. Cl., Cluster.