Figure 7
The ability of truncated NiV N proteins to interact with NiV P proteins. Cells were transfected with truncated NiV N constructs for 48 hours and protein expression was subsequently monitored from total cell lysates. A monoclonal antibody against NiV P was used to immunoprecipate NiV P proteins and co-immunoprecipate truncated NiV N proteins from cell lysates that transiently co-expressed (a) truncated NiV N proteins fused to an HA tag with NiV P proteins or (b) truncated NiV N proteins fused to GFP with NiV P proteins. Experiments were repeated at least three times. Blots have been cropped to ease visualization.
