Figure 8
Analysis of NiV replication as expression levels of truncated NiV N-HA protein increase. All experiments were carried out in triplicate and standard deviations of the mean were calculated. Cells transiently expressing increased amounts of various truncated NiV N proteins were infected with NiV at an MOI of 1 for 24 hours. Lysates were harvested from cells expressing (a) NiV N truncations fused to an HA tag or (c) NiV N truncations fused to GFP and analyzed by western blots using a monoclonal antibody against NiV P. Western blots were quantified by densitometry and normalized against the expression of actin. Expression levels of viral NiV P in the absence of recombinant NiV N was set to 100% expression. Supernatants were harvested from cells expressing (b) NiV N truncations fused to an HA tag or (d) NiV N truncations fused to GFP, viral loads were determined by endpoint titration and TCID50/ml was calculated. All experiments were done in triplicate and standard deviations of the mean were calculated.
