Figure 1
From: The PDL1-inducible GTPase Arl4d controls T effector function by limiting IL-2 production
Arl4d expression is PD-L1/PD-1 dependently regulated in CD8 T cells. (A,B) Naive OT-1 CD8 T cells were cultured for the indicated times on C57BL/6 (wild type) LSEC, Pdl1−/− LSEC or C57BL/6 (wild type) DC in the presence of antigen (100 μg/ml OVA). (A) relative Arl4d mRNA expression levels in CD8 T cells. (B) IL-2 concentration in the culture supernatant. (C) Wild type CD8 T cells were cultured in the presence or absence of coated anti-CD3ε/CD28 antibodies. After 24 h T cells were harvested and Arl4d and Il2 mRNA levels were determined by qPCR and IL-2 content in the supernatant by ELISA. The data shown are representative of 3 separate experiments. Data are shown as mean +/− s.e.m. Statistical significance was calculated using a one-way ANOVA, * p ≤ 0.05, ** p ≤ 0.01, ***p ≤ 0.001.
