Figure 3

Arl4d-deficiency does not affect T cell development, but leads to enhanced IL-2 production. (A) CD8⁺ T cells and CD19⁺ B cells were isolated from the spleen of Arl4d^−/− mice and their wild type littermate controls. qPCR was performed to determine Arl4d mRNA expression. (B) Organ weights of Arl4d^−/− mice (n = 6) and their wild type littermate controls (n = 6). (C) Total cell counts in different lymphatic (peripheral lymph node, spleen) and the bone marrow in Arl4d^−/− mice (n = 6) and their wild type littermate controls (n = 6). (D) Analysis of the T cell compartment in the spleen from 6–8 week old Arl4d^−/− mice (n = 6) and their wild type littermate controls (n = 6). Cells were gated according to CD4 or CD8 expression and according to their expression of CD44 and CD62L (T_naiv: CD44^lowCD62L^high, T_eff: CD44^highCD62L^low, T_mem: CD44^highCD62L^high). (E) Arl4d^−/− and wild type CD8 T cells were stimulated with plate-bound anti-CD3ε (1 μg/ml) and anti-CD28 (10 μg/ml) antibodies for the indicated times, after which IL-2 content in the supernatant was assed by ELISA. The data shown are representative of 3 (A) and 4 (E) separate experiments, respectively, and shown as mean +/− s.e.m. Statistical significance was calculated using a two-way ANOVA, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.