Figure 3

Shrinking of BM-derived macrophages using hyperosmolar solutions. (a) Flow cytometric analysis using FSC-H/SSC-H plot discrimination (left panel) and mean of FSC-H (right panel) for control (PBS) and hypertonic solutions (n = 4–5, biological repeats, mean ± SEM, significance of ***P < 0.001 by one-way ANOVA with Tukey’s post-hoc-test). (b) Representative brightfield images of BM-derived macrophages after incubation in control medium and 1000 mosm solution (scale bar: 200 µm) and ImageJ analysis of cell area (right panel; n = 105–482, technical repeats, mean with 95% CI, significance of ***P < 0.001 by one-way ANOVA with Tukey’s post-hoc-test). (c) Flow cytometric analysis of cell viability by propidium iodide (PI) staining of macrophages after incubation (60 min) in control (PBS) and hypertonic solutions. (d) Quantification of cell viability after incubation in control (PBS) and hypertonic solutions (n = 4–5 mean ± SEM). (n.s: denotes not significant analyzed by two-way ANOVA with Bonferroni post-hoc-test).