Figure 4

Functional analysis of BM-derived macrophages after incubation in hyperosmolar solutions. (a) Flow cytometric analysis CD45.1, CD11b and F4/80 surface marker expression on BM-derived macrophages after incubation in control (PBS) and hypertonic solutions (grey filled line: isotype, red line: surface marker). (b) Delta mean fluorescent intensity (MFI) of surface marker expression (delta MFI was calculated by subtracting isotype (negative) MFI from surface marker (positive) MFI; n = 3 biological repeats, mean ± SEM, n.s: not significant by one-way ANOVA with Tukey’s post-hoc-test). (c) Cytospins of BM-derived macrophages after incubation in control (PBS) and hypertonic solutions (scale bar: 20 µm). (d) Phagocytic activity of unsprayed and sprayed BM-derived macrophages. Left: Representative flow cytometric analysis (grey filled line: cells without S. aureus BioParticles, red line: cells with S. aureus BioParticles) and quantification of n = 3 (mean ± SEM) (n.s denotes not significant, significance of ***P < 0.001 by one-way ANOVA with Tukey’s post-hoc-test).