Figure 5
From: An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
Local administration of GFP-labeled U937 cells into isolated perfused rat lung (IPL) (a) Representative fluorescent microscopy images of U937 cells transduced with lentiviral vectors expressing GFP from a CMV early enhancer/chicken beta-actin promoter (Lv.CAG.GFP) (scale bar: 100 µm; BF: brightfield) (b) Flow cytometric analysis of GFP expression in Lv.CAG.GFP transduced U937 cells (blue line: untransduced cells, red line: cells transduced with Lv.CAG.GFP) (c) Analysis of U937 cell localization after application to the IPL. Upper panels: Immunofluorescence staining of cryostat sections generated from the lobus accessorius of the IPL (left: scale bar 50 µm, right: scale bar 10 µm), U937 cells were detected by anti-GFP antibody staining. Lower panel: Representative images of horizontal 2-Photon microscopy of lobus accessorius (right: scale bar: 60 µm).
