Figure 1
From: Aberrant DNA methylation of Tgfb1 in diabetic kidney mesangial cells
Mesangial cell fibrosis and upregulation of TGFB1 expression in db/db mice. (A) PAS staining and representative images of immunostaining of α−smooth muscle actin (α-SMA) and type IV collagen (Col IV) in glomeruli from 15-week-old db/m, db/db, Mesangial cell fibrosis and the expression of α-SMA and Col IV are significantly increased in db/db mice compared to db/m mice. (B) Real-time PCR of Tgfb1 mRNA (normalized to Gapdh) and the results of immunoblotting of TGFB1 (normalized to beta-actin) in whole kidneys of 15-week-old db/m, db/db mice. The expression of Tgfb1 mRNA and TGFB1 protein in db/db mice significantly higher than that in db/m mice. Data represent the mean ± SEM. n = 6. Filled circles: db/m mice; open circles: db/db mice. Original gel image of western blot analysis are presented in Supplemental Fig. 4.
