Figure 5
Active site properties of NOCT. (A) Accessibility for a probe of 2.5 Å radius calculated in SEQMOL-Kd. Darker areas show deeper pockets. (B) Side view (slice) of the active sites in NOCT, PDE12, CNOT6L and TDP2. All proteins were superimposed and positioned identically on the panels. TDP2 has the most open active site, whereas access to the NOCT active site is occluded by R290. (C) Recombinantly purified NOCT 122–431 WT and R290A were tested for ribonuclease activity. Reactions were carried out with 20 μM protein at 22 °C for 30 min. “M” lane shows RNA size markers.
