Figure 3
Expression of PGE2-synthesising enzymes and PGE2 levels in livers of wild-type and mPGES-1-deficient mice fed a standard- or NASH-diet. Male mPGES-1+/+ (WT) or mPGES-1−/− (KO) mice received the diets for 20 weeks. (A,B) Relative mRNA and protein expression of COX-2 (gene name Ptgs2) including representative blots in mice liver homogenates. Original blots are provided in Supplementary Figure S3. Dense intensity of COX-2 was normalized to FastGreen staining, which was verified on the same Western blot membrane as a loading control and calculated relative to the group ‘WT Standard-diet’ on each gel. (C) Relative mRNA expression of mPGES-1 (gene name Ptges). (D) PGE2 levels were determined in liver homogenates. Values are median (line), upper- and lower quartile (box) and extremes (whiskers) of 12–28 mice per group. Statistics: Two-way-ANOVA with Tukey’s post hoc test for multiple comparisons. *p < 0.05. n.d.: not detectable.
