Figure 4
From: Characterizing the “sweet spot” for the preservation of a T-cell line using osmolytes
Post-thaw recoveries of Jurkat cells cryopreserved at a cooling rate of 1 °C/min and plotted to show (a) the effect of sucrose with coloring by level of glycerol, (b) the effect of sucrose with coloring by level of isoleucine, (c) the effect of glycerol with coloring by level of sucrose, (d) the effect of glycerol with coloring by level of isoleucine, (e) the effect of isoleucine with coloring by level of sucrose, and (f) the effect of isoleucine with coloring by level of glycerol. Each solid line demonstrates the effect of the x-axis osmolyte on post-thaw recovery for fixed levels of the other two osmolytes. The dashed lines indicate the post-thaw recoveries for the single component solutions.
