Figure 3
Increased in vitro lymphoproliferative response induced by murine dendritic cells treated with GXM or GalXM. Dendritic cells (3 × 104/well) were incubated for 24 h in the presence of 50 μg/mL of the constituents’ capsule of C. neoformans. The dendritic cells were then incubated with (A) MLN cells (3 × 105/well) or with (B) MLN (3 × 105/well) in the presence of ConA (5 μg/mL). After 72 h, lymphoproliferation was analyzed by the incorporation of 3H-thymidine in the last 18 h of culture. Lipopolysaccharide (LPS) was used at a concentration of 100 ng/mL. (C) Dendritic cells were incubated with MLN cells (3 × 105/well). After 24 or 48 hours, T cells were labeled with anti-CD4 (allophycocyanin) and the percentage of apoptotic cells was quantified by flow cytometry using annexin V (FITC) and propidium iodide. Dexamethasone (1 μM) was used as a positive control for apoptosis. The results represent one of three independent experiments. The asterisks indicate ***P < 0.001 and *P < 0.05 relative to the control.
