Figure 9
IL-6−/− mice infected with C. neoformans produce increased numbers of fungal cells. Interleukin-6 deficient mice (D–F) were intratracheally pretreated with PBS (B,E) or 250 μg/mL capsular GalXM (C,F) 24 h before an intratracheal injection with 106 C. neoformans cells. After 30 days, the mice were euthanized, and the lungs were recovered. Fungal cells were identified in histological sections positive for mucicarmine. The black arrows in Panels B and C indicate C. neoformans. Panels E and F show an abundance of C. neoformans. The results represent one of three independent experiments (n = 5 mice/group). The photographs were taken under an optical microscope at 100× (A–C) and 200 × (D–F) magnification.
