Figure 3

Analysis of luminal and basal marker protein expression highlights heterogeneity of the MaSC fraction. Cells dissociated from pubertal bovine mammary tissue were sorted according to the level of expression of both CD49_f and CD24 and analyzed by immunofluorescence. (a) CD49_f^low CD24^pos cells were co-stained with anti-CD10 and -KRT8 antibodies. (b) CD49_f^high CD24^pos cells were co-stained with anti-keratin (KRT) 14 and KRT7 antibodies. Nuclei were counterstained with Hoechst 33342. Scale bars, 100 µm.