Figure 4

Gene expression levels in epithelial cell sub-populations. Cells dissociated from pubertal bovine mammary tissue were co-stained with anti-CD49f -FITC (CD49f) and anti-CD24-APC (CD24) antibodies and five sub-populations were sorted. The level of expression of the indicated genes was measured in each sub-population by RT-qPCR. Data are expressed as the mean of Delta Ct calculation ± SEM from three independent experiments (3 cows). Different letters (a–d) indicate significant differences. Abbreviations: ALDH1, Aldehyde dehydrogenase 1; ELF5, E74-like factor 5; ERα, Estradiol receptor alpha; KRT, keratin; NOTCH1, Notch homolog 1, translocation-associated; PR, Progesterone receptor; PRLR, prolactin receptor; PROCR, Protein C receptor.