Figure 10
Hydrostatic pressure (HP)-stimulated pellet cultures show increased gene expression levels of inflammatory cytokines. In comparison to the static group, HP-stimulated pellet cultures showed increased gene expression of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α on day 21. On day 42, in both HP-stimulated groups IL-1β, IL-6 and TNF-α expression was upregulated compared to unstimulated pellets. IL-6 was equally highly expressed in both HP-stimulated groups. Compared to day 0, unstimulated pellets harvested on day 21 had reduced IL-1β and IL-6 gene expression, though TNF-α was similarly expressed. Data from 3 individual donors, 5 replicates per donor; ****p < 0.0001.
