Figure 3 | Scientific Reports

Figure 3

From: Effects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish

Figure 3

Congenital anomalies in channel catfish (Ictalurus punctatus) 4-month-old fingerlings microinjected at the one cell stage with gRNAs/Cas9 protein targeting toll/interleukin 1 receptor domain-containing adapter molecule (TICAM 1) gene and rhamnose binding lectin (RBL) gene. (A) Absence of the right barbels. (B) Absence of the left eye. (C) Spinal deformity. (D) The head is curved to the left side with mouth deformity. (E,F) Normal fish. All injected and non-injected 4-month-old control fry were free of any anomalies. Photos by Ahmed Elaswad.

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