Figure 2
Organization of the 4 copies of the A2 gene on chromosome 22 in the attenuated cutaneous L. donovani strain. (a) Locations of the 4 A2 genes are shown in blue and numbered 1–4. Interspaced A2-rel genes are labeled in orange, 3′ A2-rel genes are labeled in green and 5′ A2-rel genes are labeled in yellow. A2-rel genes have no homology with A2 genes15. Transcription direction is shown according to strandedness: blue represents reverse strand direction of transcription, red represents forward strand transcription. The genes located in the 63 kb region between opposing A2 clusters are not depicted for clarity. (b) Alignment of the longest (~11 kb+) PacBio reads to the A2 clusters. Reads in the 5′ to 3′ direction labeled in red; reads in the 3′ to 5′ direction labeled in blue. (c) Western blot analysis of A2 proteins in the attenuated cutaneous L. donovani strain. The sizes of the A2 proteins are consistent with the ORFs and number of A2 genes identified in this assembly. (d) Coverage graph of chromosome 22 using Illumina (blue) and PacBio (orange) reads.
