Table 2 Summary of novel mutations identified in this study.

From: A complete Leishmania donovani reference genome identifies novel genetic variations associated with virulence

Chr	Gene	Mutation	PROVEAN	Protein Name
7	LdBPK_070700 LdCL_070011900	Ala282Val	−0.743	vacuolar-type Ca2 ± ATPase, putative
12	LdBPK_120275 LdCL_120008300	Glu1157Asp	−0.258	Myotubularin-related protein, putative
14	LdCL_140017600	Ser2919fs	N/A	kinesin k39
14	LdBPK_141190 LdCL_140017700	Glu1034Asp	−1.06	kinesin K39
22	LdBPK_220840 LdCL_220015800	Pro219F/S	N/A	hypothetical protein
23	LdCL_230017500	INS:446Glu^a	−12.453	sucrose hydrolase-like protein
25	LdBPK_250620 LdCL_250011400	Ala969Glu	0.736	Raptor N-terminal CASPase like domain containing protein
25	LdBPK_250790 LdCL_250013200	INS :110 Ala, Asn, Ser, Ala, Ala, Ala, Ala	N/A	hypothetical protein
27	LdBPK_270830 LdCL_270014900	Ala1493Thr	−0.25	ATP-binding cassette protein subfamily A
29	LdCL_290028400	Thr208Ala	0.4	VIT family putative
30¹	LdBPK_301640 LdCL_300021700	Gln334STOP^a	N/A	hypothetical protein
31	LdBPK_311390 LdCL_310020800	STOP1486Leu,Ser,His	0	hypothetical protein
31	LdBPK_311470 LdCL_310021600	Thr498Ala^a	−0.15	hypothetical protein
31	LdBPK_311470 LdCL_310021600	His497Arg^a	0.942	hypothetical protein
31	LdBPK_311470 LdCL_310021600	Gly380Asp^a	−0.383	hypothetical protein
IV → VL mutations
23	LdBPK_230830 LdCL_230014900	Asp712Glu	−1.625	hypothetical protein, unknown function
31	LdBPK_312870 LdCL_310037100	Met189Thr	−4	hypothetical protein, unknown function
31	LdBPK_313290 LdCL_310041200	Val187Phe	−0.634	Hypothetical protein
34	LdBPK_342210 LdCL_340029800	Thr116DEL	−1.098	hypothetical protein
IV-Only mutations
14	LdBPK_140470 LdCL_140010000	Gln89Lys	−0.044	cystathionine beta-lyase-like protein
31	LdBPK_312810 LdCL_310036400	Cys173Phe	−9.5	regulator of chromosome condensation (RCC1) repeat, putative
32	LdBPK_312770 LdCL_310035800	Gly667Ser	−1.292	hypothetical protein
32	LdBPK_324000 LdCL_320046000	Val250Ile	0	hypothetical protein, unknown function
36	LdBPK_361580 LdCL_360021300	Gene deletion	N/A	Serine/Threonine Kinase, putative
36	LdBPK_361590 LdCL_360021400	Gene deletion	N/A	Serine/Threonine Kinase, putative
36	LdBPK_361600 LdCL_360021500	Gene deletion	N/A	Engulfment and cell motility domain 2, putative
36	LdBPK_361610 LdCL_360021600	Gene deletion	N/A	Predicted tripartite motif protein

All mutations are annotated using VL as the wild type amino acids and CL as the mutated amino acids. Genes with annotations in the previous assembly list the previous gene ID in italic, genes annotated only in this assembly list only one gene ID. The top segment lists fifteen attenuated cutaneous strain specific mutations identified in this study. Mutations marked with^a appear at 50% but also co-occur with gene duplication event and are therefore possibly homozygous on one copy. ‘INS’ denotes amino acid insertions, ‘F/S’ denotes frameshifts, ‘DEL’ denotes amino acid deletions. The middle segment lists four mutations where the gain-of-function IV strain’s genotype changed towards that of the visceral genotype. The bottom segment lists eight mutations present only in the gain-of-function IV strain and likely represents adaptations specific to the murine host. Calculated PROVEAN scores are shown in the fourth column, scores below the −2.5 threshold for deleterious mutations are highlighted in bold²⁵.

Back to article page

Table 2 Summary of novel mutations identified in this study.

Search

Quick links