Figure 2 | Scientific Reports

Figure 2

From: Lung fibroblasts express a miR-19a-19b-20a sub-cluster to suppress TGF-β-associated fibroblast activation in murine pulmonary fibrosis

Figure 2

The miR-19a-19b-20a sub-cluster is associated with a part of TGF-β-signaling-associated genes. (A) TGF-β-signaling-pathway associated miRNAs in groups B1 and Y1 identified using DIANA-miRPath version 3.0 with Tarbase version 7.0. The number of TGF-β-signaling genes associated with each mature miRNA is shown. (B) qPCR validations of miR-19a, 19b and 20a expression in fibroblasts from bleomycin- and silica-treated murine lungs on days 7 and 14, as well as untreated mice on day 0. The relative changes in gene expression were analyzed by the 2−ΔΔCT method. qPCR and global miRNA-expression data showed similar trends related to expression kinetics. Graphs of the qPCR data show the mean ± SEM (n = 4). *P < 0.05; **P < 0.01; ***P < 0.001, one-way ANOVA with Tukey-Kramer’s multiple-comparison post hoc test. Cohen’s effect size d in these data was >0.7. (C) Details of the TGF-β signaling pathway-related genes potentially targeted with mmu-miR-19a-3p, 19b-3p, 20a-5p and 20a-3p.

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